EFFECTS OF RESVERATROL ON HISTOLOGY OF WOUND (MAST CELLS, FIBROBLASTS. COLLAGEN AND KERATINOCYTES) IN TYPE II DIABETIC WOUND HEALING OF MALE WISTAR RATS

Abstract

Background: Resveratrol contains anti-inflammatory, antidiabetic, antioxidant, anti-apoptotic,

and cytoprotective properties.

Aim: This study examines how resveratrol affects mast cells, keratinocytes, collagen, and

fibrinocytes in Male Albino Wistar Rats during wound healing.

Methods: 20 Adult Albino Male Wistars were used. High-fat diets and Alloxan injections made

rats diabetic. The rats were divided into four groups: Negative Control, Positive Control, Diabetic

Treatment Group 1 (10mg/kg resveratrol), and Diabetic Treatment Group 2 (20mg/kg). For 14

days, excisional wounds were produced and monitored for closure. Biochemical indicators,

histology, and macroscopic approaches were used to study. Biochemical, histological, and

macroscopic evidence showed wound healing.

Results: There was not statistical difference in Fasting Blood Glucose between the Diabetic

Control Group with the treatment groups. There was statistical difference between Diabetic

Control Group (210.02 ± 8.62) comparedtothegrouptreatedwithResveratrol20 mg/kg (215.43

±14.08) in the weight of the experimental animals. There was a significant acceleration in wound

closure rate, higher fibroblasts, collagen and keratinocytes presence in the diabetic untreated group

from the photomicrographs of skin wounds while the photomicrographs of skin wounds from the

diabetic wounded group treated with resveratrol (10mg) and (20 mg) showing abundance collagen

fiber in the dermis and moderate keratinocyte, the evidence collagen formation in skin and marked

granulation with more connective tissue markers.

Conclusion: Resveratrol supplement effectively reduced the fasting blood glucose and increased

the rate of wound healing as evident in the histological assessment of the treated groups with

resveratrol.