HEPATOPROTECTIVE ACTIVITY OF ETHYL ACETATE FRACTION OF BOMBAX COSTATUM (PELLEGR. ET VUILLET) STEM BARK AGAINST CARBON TETRACHLORIDE INDUCED ACUTE LIVER INJURY IN MICE

Authors

  • N. Mohammeda, A. Saleh, J. Ya’u, B.N. Umar, R.M. Hamza, N.S. Ismailf K. Usman, A. M. Musa Clinical Pharmacology and Therapeutics, Ahmadu Bello University Zaria

Keywords:

Keywords: Bombax costatum; Carbon tetrachloride; Ethyl acetate; Hepatoprotective; Necrosis

Abstract

Background: Hepatic disease is still a major public health problem due to susceptibility of
the liver to a variety of metabolic, circulatory, neoplastic, xenobiotic and microbial insults.
Bombax costatum stem bark was reported to possess hepatoprotective activity but high dose
was found to be associated with hepatocellular necrosis. The search for new effective therapy
with minimal side effect is still ongoing.
Objectives: To evaluate the hepatoprotective activity of ethyl acetate fraction of B.costatum
stem bark against carbon tetrachloride (CCl4) induced acute liver injury in mice.
Methodology: Phytochemical and acute toxicity studies of the ethyl acetate fraction of B.
costatum stem bark were carried out. Single dose of 1 ml/kg of CCl4 was administered
intraperitoneally to treated groups of mice to induce acute hepatotoxicity while in vitro 1,1
diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method was used for antioxidant
study. Effects of the ethyl acetate fraction of B.costatum on DPPH radical, serum biochemical
parameters and liver histopathology were analysed.
Results: Phytochemicals present include tannins, triterpenes and flavonoids. Oral median
lethal dose was estimated to be >5000 mg/kg. Pre-treatment of mice with the ethyl acetate
fraction of B.costatum stem bark at 62.5 and 125 mg/kg significantly (p<0.001) reduced
alanine aminotransferase (55.6±6.27 and 54.2±2.73 respectively) and aspartate transaminase
levels (76.8±5.16 and 53.2±3.44 respectively) compared to CCl4 toxic group. These results
were supported by the preservation of the liver against CCl4 -induced hepatocellular necrosis
in the ethyl acetate fraction treated groups.
Conclusion: The results obtained suggest that ethyl acetate fraction of B.costatum stem bark
possesses hepatoprotective activity against CCl4 induced acute liver injury. The
hepatoprotective effect of the ethyl acetate fraction of B.costatum stem bark could be due to
its antioxidant activity.

Author Biography

N. Mohammeda, A. Saleh, J. Ya’u, B.N. Umar, R.M. Hamza, N.S. Ismailf K. Usman, A. M. Musa, Clinical Pharmacology and Therapeutics, Ahmadu Bello University Zaria

N. Mohammed, A. Saleh, J. Ya’u, B.N. Umar, R.M. Hamza, N.S. Ismailf, K.
Usman, A. M. Musa
a= Department of Clinical Pharmacology and Therapeutics, Ahmadu Bello University Zaria
b= Department of Veterinary Pathology, Ahmadu Bello University Zaria, Nigeria
c= Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria, Nigeria
d= Department of Veterinary Microbiology, Ahmadu Bello University Zaria, Nigeria
e= Department of Human Anatomy, Federal University Dutse, Nigeria
f= Ahmadu Bello University Medical Centre, Ahmadu Bello University Zaria, Nigeria
g= Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University Zaria
*Corresponding author: Nuhu Mohammed, nuhumuhd8@gmail.com, +2348069675540

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Published

2026-08-20

Issue

Vol. 3 No. 2 (2023): Archives of Pharmaceutical Sciences and Biotechnology Journal

Section

Articles